Figure 1

Liver-specific ablation ofPtenorTsc1leads to increased activation of Akt or mTOR pathway. A) PCR genotyping illustrating the detection of Cre, and Tsc1^fl_,Pten^fl alleles in the various groups. Two of each genotype are shown. Control mice carried floxed alleles for Tsc1 and do not possess the Cre allele. B) Western blotting of liver lysates from representative mice of each genotype using antibodies directed against the indicated proteins.