Expression of functional parameters of NK cells. PBMCs were stained with antibodies and analyzed by flow cytometry to evaluate the differences of functional parameters in normal weight and obese subjects. (A) TNF-related apoptosis-inducing ligand (TRAIL) positive NK cells, (B) CD178 positive NK cells, and (C) CD107a positive NK cells as percentage of NK cells. Representative flow cytometry dot plots of CD107a from a normal weight (middle panel) and an obese individual (right panel). Inserts in both dot plots show the prior gating for NK cells (CD56+CD3−) in the upper left quadrant. Data are expressed as mean + SEM. Significant effects of obese vs. normal weight subjects are indicated by an asterisk *p < 0.05.