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Table 1 Four constructs were tested for fluorescent reporter expression during the differentiation of 3T3-L1 preadipocytes

From: Adipocyte nuclei captured from VAT and SAT

aADNp::promoted reporter protein Day 4 Day 5 Day 6 Day 7 Day 10 Day 14
mRFP1 + ++ +++ +++ +++ +++
SUN1mRFP1Flag ND ND ND + +++ +++
Nesp3mRFP1 ND ND ND ND ND +
Nesp3mRFP1Nesp ND ND ND + ++ ++
RANGAP1GFP ND ND ND ND + ++
  1. ND not detected. Standard
  2. aThe time (days) after induction to differentiate into MAs when the fluorescent protein was detected is indicated. Preadipocytes were nucleofected with five adiponectin promoted reporters, one for cytoplasmic expression (mRFP1) to test the vector pADNpcDNA3.1 KanR and four others targeting nuclei and designed to implement INTACT technology