Adipocyte nuclei captured from VAT and SAT

BMC Obesity

Table 1 Four constructs were tested for fluorescent reporter expression during the differentiation of 3T3-L1 preadipocytes

^aADNp::promoted reporter protein	Day 4	Day 5	Day 6	Day 7	Day 10	Day 14
mRFP1	+	++	+++	+++	+++	+++
SUN1mRFP1Flag	ND	ND	ND	+	+++	+++
Nesp3mRFP1	ND	ND	ND	ND	ND	+
Nesp3mRFP1Nesp	ND	ND	ND	+	++	++
RANGAP1GFP	ND	ND	ND	ND	+	++

ND not detected. Standard
^aThe time (days) after induction to differentiate into MAs when the fluorescent protein was detected is indicated. Preadipocytes were nucleofected with five adiponectin promoted reporters, one for cytoplasmic expression (mRFP1) to test the vector pADNpcDNA3.1 KanR and four others targeting nuclei and designed to implement INTACT technology

ISSN: 2052-9538