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Table 2 Recovery of adipocyte nuclei captured on immuno-paramagnetic beads from VAT, SAT, and BAT dissected from the MA-INTACT mouse

From: Adipocyte nuclei captured from VAT and SAT

Tissue Tissue weight (ave.) # nuclei recovered (ave.)/nuclei/mg # adipocyte nuclei captured ave.a/% of total
VAT 370 ± mg 93,000±/251± 41,000/44.8 ± 6 %
SAT 160 ± mg 94,000±/587± 48,000/49.5 ± 3 %
BAT 85 ± mg 44,000±/518± 30,000/63.5 ± 8 %
  1. aStandard errors computed from 5 capture experiments on 5 separate 3-month-old MA-INTACT mice using rabbit anti-mRFP1 polyclonal antibodies on 2.8 micron protein-G coupled paramagnetic Dynabeads. Similar, results were obtained when anti-RFP and anti-Flag antibodies were used as capture reagents