Adipocyte nuclei captured from VAT and SAT

BMC Obesity

Table 2 Recovery of adipocyte nuclei captured on immuno-paramagnetic beads from VAT, SAT, and BAT dissected from the MA-INTACT mouse

Tissue	Tissue weight (ave.)	# nuclei recovered (ave.)/nuclei/mg	# adipocyte nuclei captured ave.^a/% of total
VAT	370 ± mg	93,000±/251±	41,000/44.8 ± 6 %
SAT	160 ± mg	94,000±/587±	48,000/49.5 ± 3 %
BAT	85 ± mg	44,000±/518±	30,000/63.5 ± 8 %

^aStandard errors computed from 5 capture experiments on 5 separate 3-month-old MA-INTACT mice using rabbit anti-mRFP1 polyclonal antibodies on 2.8 micron protein-G coupled paramagnetic Dynabeads. Similar, results were obtained when anti-RFP and anti-Flag antibodies were used as capture reagents

ISSN: 2052-9538