The effects of dietary macronutrient composition on lipid metabolism-associated factor gene expression in the adipose tissue of chickens are influenced by fasting and refeeding

Table 2 Primers used for real time PCR

Gene^a	Primers sequence (5′–3′); Forward/Reverse	Accession No.
β-actin	GTCCACCGCAAATGCTTCTAA/TGCGCATTTATGGGTTTTGTT	NM_205518.1
PPARγ	CACTGCAGGAACAGAACAAAGAA/TCCACAGAGCGAAACTGACATC	NM_001001460.1
C/EBPα	CGCGGCAAATCCAAAAAG/GGCGCACGCGGTACTC	NM_001031459.1
C/EBPβ	GCCGCCCGCCTTTAAA/CCAAACAGTCCGCCTCGTAA	NM_205253.2
FABP4	CAGAAGTGGGATGGCAAAGAG/CCAGCAGGTTCCCATCCA	NM_204290.1
SREBP1	CATCCATCAACGACAAGATCGT/CTCAGGATCGCCGACTTGTT	NM_204126.1
KLF7	GATGCTGGTTTTCCTCACAGTTT/CCTCCTGTCCCAAAAGTGTTCA	XM_004942644.2
GATA2	CCACGAAGCAAGGCCAGAT/GGTAGCGGTTGCTCCACAGT	XM_015293080.1
AGPAT9	CCCATAGATGCGATCATTTTGA/CGTGAACTTGGCCAACCAT	NM_001031145.1
MGLL	GCAGACGAGCATAGACTCA/GGGAATAGCCTGGTTTACAA	XM_015293082.1
ATGL	GCCTCTGCGTAGGCCATGT/GCAGCCGGCGAAGGA	NM_001113291.1
CGI-58	CGCCCAGTGGTGAAAC/GCCTTTTTGCCCATCCATAA	NM_001278145.1
PLN1	GGAGGACGTGGCATGATGAC/GGCCCTTCCATTCTGCAA	NM_001127439.1
ACADL	GACATCGGCACTCGGAAGA/CCTGGTGCTCTCCCTGAAGA	NM_001006511.2
LPL	GACAGCTTGGCACAGTGCAA/CACCCATGGATCACCACAAA	NM_205282.1
NPY	CATGCAGGGCACCATGAG/CAGCGACAAGGCGAAAGTC	M87294.1
NPYR2	TGCCTACACCCGCATATGG/GTTCCCTGCCCCAGGACTA	NM_001031128.1

^aGene abbreviations: Peroxisome proliferator-activated receptor gamma: PPARγ; CCAAT/enhancer-binding protein alpha and beta: C/EBPα and C/EBPβ, respectively; Fatty acid binding protein 4: FABP4; Sterol regulatory element-binding transcription factor 1: SREBP1; Kruppel-Like Factor 7: KLF7; GATA binding protein 2: GATA2; 1-acylglycerol-3-phosphate O-acyltransferase 9: AGPAT9; Monoglyceride lipase: MGLL; Adipose triglyceride lipase: ATGL; Comparative gene identification-58: CGI-58; Perilipin 1: PLN1; Acyl-CoA dehydrogenase, long chain: ACADL; Lipoprotein lipase: LPL; Neuropeptide Y: NPY; NPY receptor 2: NPYR2

ISSN: 2052-9538