Effect1
| Area (μm) | Diameter (μm) |
|---|
Diet |
 HC | 368.5 | 20.3b
|
 HF | 442.4 | 22.4a
|
 HP | 361.4 | 20.3b
|
 SEM | 25.1 | 0.6 |
 
P-value | 0.056 | 0.02 |
Depot |
 Subcutaneous | 526.0a
| 24.6a
|
 Clavicular | 419.1b
| 22.0b
|
 Abdominal | 227.1c
| 16.4c
|
 SEM | 25.1 | 0.6 |
 
P-value | 0.0001 | 0.0001 |
 D × D | 0.96 | 0.97 |
-
1Main effects of diet (high-carbohydrate: HC; high-fat: HF; and high-protein: HP), adipose tissue depot (subcutaneous, clavicular and abdominal), and the interaction between diet (D) and depot (D) on adipocyte area and diameter. Images were analyzed using NIS-Elements Advanced Research Software (Nikon). The density, diameter, and area of all adipocytes within the field of an image were measured. Adipocytes were treated as binary objects with the restriction that measurements must exceed 100 μm2. Values represent least squares means and pooled standard errors of the means with associated P-values for each effect (HC: n = 7; HF: n = 6; HP: n = 7). Different superscipts within an effect are significantly different at P < 0.05, Tukey’s test
