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Table 5 Subcutaneous fat mRNA abundance at 4 days post-hatch

From: The effects of dietary macronutrient composition on lipid metabolism-associated factor gene expression in the adipose tissue of chickens are influenced by fasting and refeeding

Effect1 AGPAT9 FABP4 C/EBPα C/EBPβ KLF7 PPARγ MGLL SREBP1
 Diet
  HC 0.44 1.06a 0.35 1.77 1.31 0.45 2.60 0.51
  HF 0.36 1.00ab 0.32 1.40 1.32 0.50 2.21 0.43
  HP 0.36 0.84b 0.32 1.33 1.19 0.39 2.31 0.41
  SEM 0.04 0.06 0.02 0.18 0.09 0.04 0.27 0.04
   P-value 0.23 0.02 0.37 0.21 0.66 0.27 0.55 0.10
  Fed 0.57a 1.03 0.51a 1.00b 1.28b 0.73a 2.42 0.67a
  Fasted 0.31b 0.96 0.16c 2.26a 1.59a 0.32b 2.54 0.39b
  Refed 0.31b 0.91 0.33b 1.18b 0.97c 0.32b 2.18 0.33b
  SEM 0.04 0.05 0.02 0.17 0.09 0.04 0.26 0.04
   P-value 0.0001 0.35 0.0001 0.0001 0.0001 0.0001 0.57 0.0001
  D × T 0.13 0.22 0.003 0.31 0.77 0.21 0.67 0.06
Effect1 GATA2 ATGL PLN1 CGI-58 ACADL NPY NPYR2 LPL
 Diet
  HC 0.97 1.41 0.89 1.24 0.70 0.91 0.69 1.21
  HF 1.05 1.42 0.77 0.70 0.56 0.64 0.66 1.41
  HP 0.86 1.36 0.87 0.96 0.49 1.09 0.61 1.64
  SEM 0.07 0.17 0.13 0.19 0.15 0.26 0.05 0.19
   P-value 0.11 0.96 0.76 0.15 0.57 0.46 0.48 0.30
Treatment
 Fed 1.10 1.50 0.79 1.20 0.72 1.35 0.89a 1.55
 Fasted 0.90 1.38 1.00 0.93 0.76 0.85 0.56b 1.25
 Refed 0.89 1.31 0.74 0.77 0.28 0.44 0.50b 1.47
 SEM 0.06 0.17 0.13 0.19 0.15 0.26 0.05 0.19
P-value 0.08 0.76 0.30 0.29 0.03 0.07 0.0001 0.50
 D × T 0.26 0.76 0.56 0.51 0.14 0.76 0.03 0.37
  1. 1Values represent least squares means and pooled standard errors of the means with associated P-values for each effect. D × T: interaction between diet (D) and treatment (T). Different superscipts within an effect for each gene are significantly different at P < 0.05, Tukey’s test. Abbreviations: 1-acylglycerol-3-phosphate O-acyltransferase 9: AGPAT9; Fatty acid binding protein 4: FABP4; CCAAT/enhancer-binding protein alpha and beta: C/EBPα and C/EBPβ, respectively; Krüppel-like factor 7: KLF7; Peroxisome proliferator-activated receptor gamma: PPARγ; Monoglyceride lipase: MGLL; Sterol regulatory element-binding transcription factor 1: SREBP1; GATA-binding protein 2: GATA2; Adipose triglyceride lipase: ATGL; Perilipin 1: PLN1; Comparative gene identification-58: CGI-58; Acyl-CoA dehydrogenase, long chain: ACADL; Neuropeptide Y: NPY; NPY receptor 2: NPYR2; Lipoprotein lipase: LPL