Fig. 5

NK cells, MADB106 cells and NK cell-MADB106 cell interactions in lung after short-term tumor challenge. a-c: Numbers of counted (a) NK cells, (b) MADB106 cells and (c) NK cell-MADB106 cell interactions in the lung after tumor challenge with MADB106 cells or NaCl for 15 min in normal weight (control) or DIO fed rats (DIO); values represent means ± SEM, n = 8 rats/group.* indicates significant differences of means (p ≤ 0.05) compared to control/MADB106 analyzed by one-way ANOVA. d-f: Double-immunostained and hematoxylin-counterstained cryostat section; staining was performed using anti-CD 161 mAb and anti-Fluorescein mAb, the slices were scanned via digital microscope and analyzed with the ImageJ software: d NK cells are stained red; e MADB106 cells are stained blue; f NK cell-MADB106 cell interactions; black arrows mark NK cells, red arrows mark MADB106 cells